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KMID : 1140120100150010052
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Cancer Prevention Research
2010 Volume.15 No. 1 p.52 ~ p.59
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The Extracts of Sargassum fulvellum Induce Pro-Apoptosis and Cell Cycle Arrest on MDA-MB-231 Human Breast Carcinoma Cells
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Yoon Jin-Soo
Woo Hee-Chul
Kim Hyeung-Rak
Kim Gun-Do
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Abstract
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The potential anti-cancer effects of n-hexane (HSF) and dichloromethane fractions (DSF) of a brown macro-algae, Sargassum fulvellum were evaluated in estrogen receptor-negative MDA-MB-231 human breast cancer cells. The effects of HSF and DSF on pro-apoptosis was assessed by studying the release of cytochrome c and the level of Bcl-xL which related with anti-adhesive effects. Cell cycle controlling proteins which associated with the level of CDK6 and phosphorylated cdc2 caused cell cycle arrest. The exposure of MDA-MB-231 cells to each fraction (HSF and DSF respectively) at concentration of 50 μg/ml for 24 hours resulted in the inhibition of cell growth and shrinking of cell morphology along with decreased expression of Rac and MAP kinases. The results suggested that the anti-cancer effects of two extracts act on pro-apoptosis, cell cycle arrest and the inhibition of cell growth.
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KEYWORD
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Sargassum fulvellum, Brown macro-algae, MDA-MB-231, Apoptosis, Cell cycle
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